Objective LD LCI Plan-Apochromat 40x/1.2 Imm Corr DIC M27
Objective LD LCI Plan-Apochromat 40x/1.2 Imm Corr DIC M27
Item no.: 420862-9970-799Objective LD LCI Plan-Apochromat 40x/1.2 Imm Corr DIC M27 for water, silicon oil or glycerine immersion (CG=0.15-0.19mm) (FWD=0.41mm at CG=0.17mm) incl. Immersol G, bottle 20 ml and Cover glasses, high performance, CG=0.17mm, box with 100 pc With serial number. Strehl ratio >90%.
Full DescriptionThis objective has a high Strehl ratio, especially for Superresolution Microscopy.
The rating of the option Definite Focus.2 is valid for use with water immersion.
Magnification | 40x |
Numerical Aperture | 1.2 |
Free Working Distance [mm] | 0.41 at cover glass 0.17 |
Coverglass Thickness [mm] | 0.15 - 0.19 |
Thread Type | M27x0.75 |
Immersion | Water, W 2010, Silicone oil and Glycerine |
Field of View [mm] | 20 |
Parfocal Length [mm] | 45.06 |
Long Distance | |
Correction Ring | |
Iris | |
Optical System | Infinity Color Corrected System (ICS) |
Color Correction | apochromatic |
Biomedical Applications | |
---|---|
Fluorescence | |
- Multichannel | |
- Ultraviolet Transmission | |
- Infra Red Transmission | |
BrightField | |
DIC [Differential Interference Contrast] | |
High Contrast DIC | |
PlasDIC | |
Phase Contrast | |
VAREL Contrast | |
Hoffman Modulation Contrast | |
Polarization Contrast | |
Materials (Reflected Light) Applications | |
BrightField | |
BrightField/DarkField | |
Reflected Light DIC | |
High Contrast DIC | |
DIC with circular polarized light | |
Total Interference Contrast | |
Polarization Contrast | |
Options | |
Definite Focus.2 | |
Confocal Microscopy | |
- Ultra Violet | |
- VIS (visible light) | |
- NLO-IR / 2 Photon | |
Total Internal Reflection Fluorescence | |
Apotome | |
Microdissection |
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Objective ring ACR for objective sleeve conical long
Item no.: 424510-0000-000Objective ring ACR for objective sleeve conical long
Item no.: 424510-0000-000Objective ring ACR for objective sleeve conical long
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DIC slider LD LCI PApo 40x/1,2 III
Item no.: 426947-9000-000DIC slider LD LCI PApo 40x/1,2 III
Item no.: 426947-9000-000DIC slider LD LCI PApo 40x/1,2 III
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Immersion medium Immersol W 2010, oiler 20 ml
Item no.: 444969-0000-000Immersion medium Immersol W 2010, oiler 20 ml
Item no.: 444969-0000-000Immersion medium Immersol W 2010, oiler 20 ml ne=1.3339 (23°C) for water immersion
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Immersion medium Immersol Sil 406, Oiler 20 ml
Item no.: 444971-9000-000Immersion medium Immersol Sil 406, Oiler 20 ml
Item no.: 444971-9000-000Immersion medium Immersol Sil 406, Oiler 20 ml Brown glass bottle 20 ml, ne=1.406 (23 °C), halogen free
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Immersion medium Immersol G, oiler 20 ml
Item no.: 462959-9901-000Immersion medium Immersol G, oiler 20 ml
Item no.: 462959-9901-000Immersion medium Immersol G, oiler 20 ml ne = 1.456 (23 °C) ve = 58 Glycerine immersion for fluorescence microscopy
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Box of lens cleaning paper (300 sheets)
Item no.: 462975-0000-000Box of lens cleaning paper (300 sheets)
Item no.: 462975-0000-000Box of lens cleaning paper (300 sheets)
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Cover glasses, high performance, D=0.17mm, box with 1000 pc.
Item no.: 474030-9000-000Cover glasses, high performance, D=0.17mm, box with 1000 pc.
Item no.: 474030-9000-000Cover glasses, high performance, D=0.17mm, box with 1000 pc. Size 18x18 mm² type 1 1/2 H as per ISO 8255-1 with restricted thickness-related tolerance D=0.17 mm +/- 0.005 mm refractive index = 1.5255 +/- 0.0015, Abbe number = 56 +/-2 recommended for applications with high numerical aperture objectives
Note:
All measures in [mm]
mech. Arbeitsabstand = mechanical working distance
Deckglas = cover glass
Objektebene = object plane
Objektfeld = object field
Ausleuchtung = illumination
Probenzugänglichkeit = specimen accessibility
Note:
Please note that due to production tolerances, the given values are typical only and not guaranteed.
These objectives have been specifically designed for Life Cell Imaging techniques and are designed for the temperature interval between 23° and 37°C. The 25x/0.8 can be used with the immersion mediums water, glycerine and oil. A single correction collar permits simple and convenient compensation for spherical aberration caused, for example, by different cover slip thicknesses, temperature drift or immersion media with a different refractive index, adjustable between water and glycerine. For the examination of living objects, water is particularly suitable as immersion medium. Furthermore, cleaning is extremely easy. For critical fluorescence examinations, cleaned glycerol is the ideal immersion medium, as it has very low autofluorescence. Therefore, glycerol and water should be preferred for the microscopy of living objects, since these are found in a medium with a similar refractive index. The Refractive Index Mismatch indicates the deviation of the actually available refractive index from the one that the objective has been calculated for. This deviation too can be compensated for by means of the correction collar. Used with inverted microscopes, multi-immersion objectives are ideal for the limited compensation of non-standard glass bottom thicknesses (D < 0,17) or the popular use of water as immersion medium with a slightly lower numerical aperture than oil-immersion objectives.
Special emphasis has been placed on good accessibility of the optical axis with micromanipulators.
Objective LD LCI Plan-Apochromat 40x/1.2 Imm Corr DIC M27 for water, silicon oil or glycerine immersion (CG=0.15-0.19mm) (FWD=0.41mm at CG=0.17mm) incl. Immersol G, bottle 20 ml and Cover glasses, high performance, CG=0.17mm, box with 100 pc With serial number. Strehl ratio >90%.