Carl Zeiss Microscopy, LLC - Objective Assistant - Objective LCI Plan-Neofluar 63x/1.3 Imm Corr DIC M27

Objective LCI Plan-Neofluar 63x/1.3 Imm Corr DIC M27

Item no.: 420882-9971-000

Description

Objective LCI Plan-Neofluar 63x/1.3 Imm Corr DIC M27 for water, silicone oil or glycerine immersion (CG=0.15-0.19mm) (FWD=0.17mm at CG=0.17mm) incl. Immersol G, bottle 20 ml and Cover glasses, high performance, CG=0.17mm, box with 100 pc.

Full Description

The rating of the option Definite Focus.2 is valid for use with glycerine immersion.

Biomedical Applications
Magnification	63x
Numerical Aperture	1.3
Free Working Distance [mm]	0.17 at cover glass 0.17
Coverglass Thickness [mm]	0.15 - 0.19
Thread Type	M27x0.75
Immersion	Water, W 2010, Silicone oil and Glycerine
Field of View [mm]	20
Parfocal Length [mm]	45.06
Long Distance
Correction Ring
Iris
Optical System	Infinity Color Corrected System (ICS)
Color Correction	semi-apochromatic
Fluorescence
- Multichannel
- Ultraviolet Transmission
- Infra Red Transmission
BrightField
DIC [Differential Interference Contrast]
High Contrast DIC
PlasDIC
Phase Contrast
VAREL Contrast
Hoffman Modulation Contrast
Polarization Contrast
Materials (Reflected Light) Applications
BrightField
BrightField/DarkField
Reflected Light DIC
High Contrast DIC
DIC with circular polarized light
Total Interference Contrast
Polarization Contrast
Options
Definite Focus.2
Confocal Microscopy
- Ultra Violet
- VIS (visible light)
- NLO-IR / 2 Photon
Total Internal Reflection Fluorescence
Apotome
Microdissection

Objective heater 33/38 S1 (D)

Item no.: 411860-9067-000

Objective heater 33/38 S1 (D)
Item no.: 411860-9067-000

Objective heater 33/38 S1 (D) - with oil discharge channel - clamping via front dia. 33 mm - max. objective dia. 38 mm - temperature range of room temperature up to +45°C
Objective ring ACR for objective sleeve conical long

Item no.: 424510-0000-000

Objective ring ACR for objective sleeve conical long
Item no.: 424510-0000-000

Objective ring ACR for objective sleeve conical long
DIC slider LCI PN 63x/1.30 III

Item no.: 426959-0000-000

DIC slider LCI PN 63x/1.30 III
Item no.: 426959-0000-000

DIC slider LCI PN 63x/1.30 III
Immersion medium Immersol W 2010, oiler 20 ml

Item no.: 444969-0000-000

Immersion medium Immersol W 2010, oiler 20 ml
Item no.: 444969-0000-000

Immersion medium Immersol W 2010, oiler 20 ml ne=1.3339 (23°C) for water immersion
Immersion medium Immersol Sil 406, Oiler 20 ml

Item no.: 444971-9000-000

Immersion medium Immersol Sil 406, Oiler 20 ml
Item no.: 444971-9000-000

Immersion medium Immersol Sil 406, Oiler 20 ml Brown glass bottle 20 ml, ne=1.406 (23 °C), halogen free
Immersion medium Immersol G, oiler 20 ml

Item no.: 462959-9901-000

Immersion medium Immersol G, oiler 20 ml
Item no.: 462959-9901-000

Immersion medium Immersol G, oiler 20 ml ne = 1.456 (23 °C) ve = 58 Glycerine immersion for fluorescence microscopy
Box of lens cleaning paper (300 sheets)

Item no.: 462975-0000-000

Box of lens cleaning paper (300 sheets)
Item no.: 462975-0000-000

Box of lens cleaning paper (300 sheets)
Cover glasses, high performance, D=0.17mm, box with 1000 pc.

Item no.: 474030-9000-000

Cover glasses, high performance, D=0.17mm, box with 1000 pc.
Item no.: 474030-9000-000

Cover glasses, high performance, D=0.17mm, box with 1000 pc. Size 18x18 mm² type 1 1/2 H as per ISO 8255-1 with restricted thickness-related tolerance D=0.17 mm +/- 0.005 mm refractive index = 1.5255 +/- 0.0015, Abbe number = 56 +/-2 recommended for applications with high numerical aperture objectives

Note:

All measures in [mm]

mech. Arbeitsabstand = mechanical working distance
Deckglas = cover glass
Objektebene = object plane
Objektfeld = object field
Ausleuchtung = illumination
Probenzugänglichkeit = specimen accessibility

Note:

Please note that due to production tolerances, the given values are typical only and not guaranteed.

LCI Plan-Neofluar

These objectives have been specifically designed for Life Cell Imaging techniques and are designed for the temperature interval between 23° and 37°C. The 25x/0.8 can be used with the immersion mediums water, glycerine and oil, while the 63x/1.3 can be used with water and glycerine. A single correction collar permits simple and convenient compensation for spherical aberration caused, for example, by different cover slip thicknesses, temperature drift or immersion media with a different refractive index, adjustable between water and glycerine. For the examination of living objects, water is particularly suitable as immersion medium. Furthermore, cleaning is extremely easy. For critical fluorescence examinations, cleaned glycerol is the ideal immersion medium, as it has very low autofluorescence. Therefore, glycerol and water should be preferred for the microscopy of living objects, since these are found in a medium with a similar refractive index. The "Refractive Index Mismatch" indicates the deviation of the actually available refractive index from the one that the objective has been calculated for. This deviation too can be compensated for by means of the correction collar. Used with inverted microscopes, multi-immersion objectives are ideal for the limited compensation of non-standard glass bottom thicknesses (D < 0,17) or the popular use of water as immersion medium with a slightly lower numerical aperture than oil-immersion objectives.
Special emphasis has been placed on good accessibility of the optical axis with micromanipulators.